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KMID : 0545119950050060364
Journal of Microbiology and Biotechnology
1995 Volume.5 No. 6 p.364 ~ p.369
Enhanced Essential Oil Formation by Two-phase Culture of Mentha piperita Cells in Shake Flask and Air-lift Bioreactors
Kim, Teresa
Kim, Tae Yong/Bae, Geun Won/Chae, Young Am/Lee, Hyong Joo/Chung, In Sik
Abstract
Effect of two-phase culture on Mentha piperita cell growth and essential oil formation was investigated using shake flask and air-lift bioreactors. LiChroprep RP-8(RP-8) addition did not impair M. piperita cell growth, but resulted in stimulated formation of essential oils and increased ratios of extracellular oil to intracellular oil formation. However, the combined use of RP-8 and chitosan elicitor was not synergistic. Volumetric productivity of essential oils in RP-8 treated culture using cell-recycled air-lift bioreactor was 6.9 §¶/§¤¡¤day which was substantially higher than that obtainable from the control. Our results demonstrate the potential of a second phase to enhance overall productivity for M. piperita cell culture.
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